Journal: Pharmaceuticals

Article Title: New Genetic Bomb Trigger: Design, Synthesis, Molecular Dynamics Simulation, and Biological Evaluation of Novel BIBR1532-Related Analogs Targeting Telomerase against Non-Small Cell Lung Cancer

doi: 10.3390/ph15040481

Figure Lengend Snippet: Telomerase activity in cancer cells as a percent of control cells with target compounds 29a , 36b , and 39b .

Article Snippet: The human A549 (epithelial cell lung carcinoma, ATCC, Manassas, VA, USA), HCC44 (non-small cell lung adenocarcinoma, Leibniz Institute DSMZ-German Collection of the Microorganisms and the Cell Cultures, Braunschweig, Germany), and NCI-H23 (non-small cell lung adenocarcinoma, ATCC, Manassas, VA, USA) cell lines (compounds 29a , 36b , and 39b ) were diluted to 10 μM and incubated for 48 h before being tested using the TRAP method.

Techniques: Activity Assay, Control

Journal: Frontiers in Microbiology

Article Title: Bacteria Are New Targets for Inhibitors of Human Farnesyltransferase

doi: 10.3389/fmicb.2021.628283

Figure Lengend Snippet: The agar well diffusion assay showed the effect of lonafarnib and tipifarnib on the formation of inhibition zones in cultures of different bacterial strains. Control (DMSO), lonafarnib, and tipifarnib (500 μM) were applied to agar plates inoculated with different bacterial strains [ (A) S. aureus , (B) MRSA, (C) S. epidermidis , (D) E. faecium , (E) E. coli , (F) K. pneumoniae , and (G) P. aeruginosa ]. Statistical significance was indicated as follows: ** p < 0.01 and *** p < 0.001. Significant differences between lonafarnib- and tipifarnib-treated S. aureus , MRSA, and S. epidermidis is indicated as follows: ***. n.s. = not significant.

Article Snippet: The following bacterial strains were used: S. aureus (DSM-799, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures), methicillin-resistant Staphylococcus aureus (MRSA) (clinical isolate, kindly provided by B. Ghebremedhin, Helios University Medical Centre, Wuppertal, Germany), Streptococcus pneumoniae ( S. pneumoniae ) (kindly provided by A. Ehrhardt, Witten/Herdecke University, Witten, Germany), E. coli (DSM-11250, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures), Pseudomonas aeruginosa ( P. aeruginosa ) (DSM-939, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures), Staphylococcus epidermidis ( S. epidermidis ) (DSM-20044, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures), Klebsiella pneumoniae ( K. pneumoniae ) (DSM-30104, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures), and Enterococcus faecium ( E. faecium ) (DSM-2146, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures).

Techniques: Diffusion-based Assay, Inhibition, Control

Journal: Frontiers in Microbiology

Article Title: Bacteria Are New Targets for Inhibitors of Human Farnesyltransferase

doi: 10.3389/fmicb.2021.628283

Figure Lengend Snippet: The time kill assay revealed the reduction rate of different bacterial strains after treatment with lonafarnib (500 μM) and tipifarnib (500 μM). The effect on (A) S. aureus , (B) MRSA , (C) S. epidermidis , (D) E. faecium , (E) S. pneumoniae , (F) E. coli , (G) P. aeruginosa , and (H) K. pneumoniae was evaluated. Statistical significance was indicated as follows: * p < 0.05; ** p < 0.01; and *** p < 0.001.

Article Snippet: The following bacterial strains were used: S. aureus (DSM-799, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures), methicillin-resistant Staphylococcus aureus (MRSA) (clinical isolate, kindly provided by B. Ghebremedhin, Helios University Medical Centre, Wuppertal, Germany), Streptococcus pneumoniae ( S. pneumoniae ) (kindly provided by A. Ehrhardt, Witten/Herdecke University, Witten, Germany), E. coli (DSM-11250, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures), Pseudomonas aeruginosa ( P. aeruginosa ) (DSM-939, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures), Staphylococcus epidermidis ( S. epidermidis ) (DSM-20044, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures), Klebsiella pneumoniae ( K. pneumoniae ) (DSM-30104, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures), and Enterococcus faecium ( E. faecium ) (DSM-2146, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures).

Techniques: Time-Kill Assay

Journal: Journal of Functional Biomaterials

Article Title: Cytocompatibility Assessment of L-PBF-Manufactured Zinc–Silver–Copper Alloys for Customized Biodegradable Medical Implants

doi: 10.3390/jfb17030146

Figure Lengend Snippet: ( A ) Representative images of the direct cytocompatibility contact test with SAOS-2 cells are presented. The top row ( a ) shows the tested sample plates (black) at the top of the image, with cells growing in direct contact on the bottom of the six-well plate (five times magnification). ( b ) The cell density at a 12 mm distance from the test plates (ten times magnification). ( B , D ) Three different extract concentrations (undiluted, diluted 1:3 and diluted 1:10) from the three L-PBF-manufactured Zn alloys (ZnAgCu, ZnAgCuMn, and ZnAgCuTi), rolled Zn, Cu, and Ti controls were tested, and the proliferation of L929 ( B ) and SAOS-2 ( D ) cells was determined after 24 h. ( C , E ) Live/dead staining of L929 ( C ) and SAOS-2 ( E ) cells growing directly on top of the samples is shown. Red and yellow staining indicate dead or dying cells, respectively, while living cells appear green. Each test was carried out four times with four replicates per sample. Shown are the mean values with their corresponding standard deviations. All results were normalized to control cells grown in cell culture medium without extract (proliferation equals 100%). To calculate statistical significance, a one-way ANOVA followed by a Tukey test was carried out (** p < 0.01 and **** p < 0.0001).

Article Snippet: In addition, the human primary osteogenic sarcoma cell line SAOS-2 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), cultured in McCoy’s 5A medium (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) supplemented with 15% FBS (Bio&SELL GmbH) and 1% L-glutamine (Thermo Fisher Scientific Inc.), was used.

Techniques: Staining, Control, Cell Culture

Journal: Journal of Functional Biomaterials

Article Title: Cytocompatibility Assessment of L-PBF-Manufactured Zinc–Silver–Copper Alloys for Customized Biodegradable Medical Implants

doi: 10.3390/jfb17030146

Figure Lengend Snippet: Time dependency of ion release and cytocompatibility of undiluted extracts tested for 24 h with SAOS-2 cells from untreated ( A ) and polished ( B ) L-PBF-manufactured plates. Extracts collected daily over a period of 10 days. ( C ) OM of untreated and polished plates before and after 10 days of immersion. The mean proliferation values with the corresponding standard deviation of independent experiments ( n = 4) are plotted graphically. All results were normalized to the control cells grown in cell culture medium without extract (=100%). Significance was determined using one-way ANOVA followed by a Tukey test (* p < 0.05; ** p < 0.01; *** p < 0.001; and **** p < 0.0001). ( D , E ) ICP-OES ion concentration measurement. ( D ) Zn 2+ release of the different alloys into daily renewed McCoy’s medium from day 1 to day 10. The extracts were taken from untreated and freshly polished samples. The mean values with the corresponding standard deviations of five independent experiments are plotted. ( E ) Ion concentrations of Zn 2+ , Ag + , Cu 2+ , and Mn 2+ release of ZnAgCuMn into the daily changed McCoy’s medium ( n = 5).

Article Snippet: In addition, the human primary osteogenic sarcoma cell line SAOS-2 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), cultured in McCoy’s 5A medium (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) supplemented with 15% FBS (Bio&SELL GmbH) and 1% L-glutamine (Thermo Fisher Scientific Inc.), was used.

Techniques: Standard Deviation, Control, Cell Culture, Concentration Assay

Journal: Journal of Functional Biomaterials

Article Title: Cytocompatibility Assessment of L-PBF-Manufactured Zinc–Silver–Copper Alloys for Customized Biodegradable Medical Implants

doi: 10.3390/jfb17030146

Figure Lengend Snippet: Cytocompatibility extract test with SAOS-2 cells cultivated for 24 h in extracts from untreated, new polished and aged polished samples. The mean proliferation values with the corresponding standard deviation of independent experiments ( n = 8) are plotted graphically. All results were normalized to the control cells grown in cell culture medium without extract (=100%). Significance was determined using one-way ANOVA followed by a Tukey test (* p < 0.05; ** p < 0.01).

Article Snippet: In addition, the human primary osteogenic sarcoma cell line SAOS-2 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), cultured in McCoy’s 5A medium (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) supplemented with 15% FBS (Bio&SELL GmbH) and 1% L-glutamine (Thermo Fisher Scientific Inc.), was used.

Techniques: Standard Deviation, Control, Cell Culture